Widoczny [Schowaj] Abstrakt. Renewable and Sustainable Energy Reviews , vol. Journal of Industrial Microbiology and Biotechnology , vol. Bioresource Technology ,vol. Bioresource Technology , vol. Journal of Analytical and Applied Pyrolysis , vol.
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Wenger, S. Senft, J. Comparison of established cell lines at different passages by karyotype and comparative genomic hybridization. Bioscience Reports, 24 6 , Won, E-J. Expression of three novel cytochrome P CYP and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction WAF of Iranian crude oil and Benzo[a]pyrene. Marine Environmental Research, 90, The aim of the study was to compare the cytotoxic action of selected second generation biofuels water-soluble fraction WSF and susceptibility of old and young passages of human diploid fibroblasts.
The MTT test determining metabolic activity and the NRU test, evaluating the integrity of cell membranes were used to assess the cytotoxic activity of tested biofuels WAF. The most cytotoxic reaching the lowest IC50 values for both types of cells was the water extract of biofuel obtained from animal waste BPII concerning the highest concentration of fatty acid esters.
Saturated fatty acids, such as capric, lauric, stearic and palmitic acids were dominant in its composition. In turn, the least cytotoxic was water fraction of the biofuel obtained from crude rapeseed oil BPIV with the lowest content of fatty acid esters, mainly consisting of monounsaturated acids: octadecenoic acid and eicosenoic acid.
Young passages of CCDSk cells were more insusceptible to the cytotoxic effects of the tested biofuels. The cytotoxicity of the most toxic and the least toxic water fraction of the tested biofuels was a reflection of the content of fatty acid esters in them.
The results indicate that the susceptibility of senescent cells to the cytotoxic action of biofuels WSF may change during their senescence — the older cells become more sensitive to the cytotoxic effect of tested xenobiotics as compared to younger cells in the most cases.
The key role in the cytotoxic action of compounds on the younger and older cell passages may also have a concentration of tested compounds. Tom 20, cz.
Chapter 3 Biofuels in Storage and Operating Conditions